Search results for " molecular marker"
showing 10 items of 29 documents
Molecular markers and endometrial cancer
2022
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Crocus sativus L. Ecotypes from Mediterranean Countries: Phenological, Morpho-Productive, Qualitative and Genetic Traits
2021
The characterization of C. sativus ecotypes is of great interest for preserving them from a possible genetic erosion due to the decrease of European cultivation surface. In this study, we evaluated four ecotypes from Italy (Sardinia and Abruzzo), Spain (Castilla-La Mancha), and Greece (Kozani) in order to detect the existence of variability and promote the biodiversity of this crop. Thirty-one traits related to saffron flowering, flower morphology, production of spice and daughter corms, vegetative development (leaf and corm traits), and spice quality, were evaluated. In addition, a genetic analysis through three PCR-based approaches, SSRs, RAPD, and SRAP was assessed. Results highlighted a…
New polymorphic markers for genetic diversity studies in an invasive plant: the common ragweed (Ambrosia artemisiifolia L.)
2014
Common ragweed is an annual herb that belongs to the Asteraceae family. It is known as an invasive plant originating from the USA. In France the common ragweed is especially abundant in the Rhône-Alpes basin and currently spreads northwards in Burgundy. Ragweed colonizes different types of environments, such as railways, river sides, wastelands, farmlands and cultivated crops (especially sunflowers crops). Herbicide resistance to linuron, glyphosate and/or acetolactate synthase inhibitors has been reported in the USA, which complicates the chemical control of ragweed. In addition, the highly allergenic pollen of ragweed causes severe allergies. These elements make this plant a major threat …
The TP53 colorectal cancer international collaborative study on the prognostic and predictive significance of p53 mutation: influence of tumor site, …
2005
Purpose The aims of the TP53 Colorectal Cancer (CRC) International Collaborative Study were to evaluate the possible associations between specific TP53 mutations and tumor site, and to evaluate the prognostic and predictive significance of these mutations in different site, stage, and treatment subgroups. Patients and Methods A total of 3,583 CRC patients from 25 different research groups in 17 countries were recruited to the study. Patients were divided into three groups according to site of the primary tumor. TP53 mutational analyses spanned exons 4 to 8. Results TP53 mutations were found in 34% of the proximal colon tumors and in 45% of the distal colon and rectal tumors. They were assoc…
A contribution to the phylogeny and biogeography of the genus Isoetes (Isoetaceae, Lycopodiidae) in the Mediterranean region
2019
To better understand the phylogeny of Isoetes species in the Mediterranean region, sequences of the second intron of a LFY-like homolog were determined for six populations of three Mediterranean Isoetes species and analyzed with other LFY and ITS sequences of Euro-Mediterranean species available in GenBank. Nomenclature of selected species was updated according to recent literature. Investigations of voucher specimens from previously sequenced taxa revealed that a specimen of I. histrix from Crete, Greece used in several previous molecular studies is probably I. phrygia. Analysis of the limited LFY and ITS DNA sequence data presented here agrees with the organization of Mediterranean specie…
Genetic Diversity of Mute Swan Population of the Riga Urban Area
2015
Abstract Mute swan Cygnus olor is the most abundant breeding swan species in Rīga and Latvia. Since manmade habitats are the preferred breeding habitats of this species - Cygnus olor is of particular interest in studies of urban biodiversity. The latest records show that 30-40 pairs breed annually in Rīga, 400-700 individuals migrate through the area, and 120-150 individuals stay over winter. The goal of our study was to determine the genetic variation of the mute swan population in Rīga. Blood samples were collected from 47 individual birds found in different sites in Rīga or elsewhere in Latvia. The universal retrotransposon based iPBS markers were used to estimate diversity. Three primer…
Somatic embryogenesis through in vitro anther culture ofCitrus sinensisL. Osbeck ‘Moro’
2019
In many crops, anther culture is the most used method to induce gametic embryogenesis, aimed to regenerate homozygous plants. However, also somatic embryogenesis can be obtained by this method, when somatic tissue is involved in regeneration process. Many factors can affect this procedure, such as genotype, pre-treatments applied to floral buds, pollen developmental stage, donor plant states, culture media composition and growth culture conditions. Anthers of Citrus sinensis L. Osbeck, cv. Moro, were collected at the vacuolate stage, and after a chilling (4°C) pre-treatment of 7 days, were placed on the same medium, evaluating different temperature stresses applied after the placing in cult…
Molecular approach for the characterization of ancient /degraded Cyperus sp.specimens
2013
The aim of this work has been to apply and improve the efficiency of molecular tools for the identification of Cyperus sp. from difTerent sources. Total DNA was extracted from fresh, degraded and ancient specimens. DNA molecules were the template for in vitro amplification of specific target regions referred to rcbL plastid gene (ribulose-l,5 -biphosphate carboxylase/oxygenase large subunit). Dedicated DNA extraction and amplification protocols have been applied specifically for fresh/degraded and ancient samples. Particularly, specific set of primer, annealing temperatures and reaction mixtures have been performed. The PCR products, single DNA fragments, were resolved on agarose gel electr…
“Identification of molecular and genetic markers common to multifactorial diseases (cardiovascular diseases, metabolic disorders, tumors) associated …
Molecular characterization of the Sicilian goat populations
2014
The Sicilian goats are characterized by a strong population admixture structure, caused by geographical location of the farms, influences of natural mating and traditional breeding systems where flock represents an important breeding unit. Nowadays, several local populations are reared in Sicily, some of which do not have a recognized defined genetic structure, having taken origin from several crosses between animals of different breeds/populations sharing the same environment. The aim of this work was to characterize the Sicilian autochthonous goat populations using microsatellite markers and genetic polymorphisms of casein genes